Topic ID #30431 - posted 12/19/2013 5:25 PM

Steroidal biomarker analysis of a 14,000 years old putative humancoprolite from Paisley Cave, Oregon



Charlie Hatchett

 Steroidal biomarker analysis of a 14,000 years old putative humancoprolite from Paisley Cave, Oregon 

Lipid components of a putative human coprolite sample from the Paleoindian site of Paisley Cave, Oregon (12,300 14C yr BP) were analyzed using GC/MS to explore its origin in light of controversial data obtained from mitochondrial DNA, cross-immunoelectrophoresis, trisodium phosphate rehydration, and micromorphology analyses. Results show the predominant presence of 5b-stigmastanol, the biomarker of herbivore fecal matter, supporting micromorphological identification of the coprolite as herbivore. This study highlights the potential of the biomarker approach in coprolite studies devoted to research on the first presence of humans in North America. 

https://www.academia.edu/

Charlie Hatchett 

Pre-Clovis Artifacts from Central Texas 
http://www.pre-clovis.com
http://www.forum.pre-clovis.com
http://www.blog.pre-clovis.com




Post ID#20368 - replied 12/21/2013 6:10 AM



Charlie Hatchett

Rick Baudé Wrote:

-------------------------------------------------------

> I'm a little confused here Charlie, since you're

> one of the few people on the board that know

> something about ' pre-clovis are they saying that

> the results are inconclusive about whether it's a

> hominid or that it leans toward a hominid origin?

 

This statement in the paper sums up the authors' view concerning one of the coprolites:

 

"...the predominant presence of 5b-stigmastanol, the biomarker of

herbivore fecal matter, supporting micromorphological identification

of the coprolite as herbivore..."

 

As I noted above:

 

“…The sample in question is 1374-5/5D-31-2. mtDNA Hg B2 was determined by Copenhagen SNaPshot, Copenhagen cloned, Uppsala Pyrosequenced, Uppsala cloned and sequenced. The sample was dated to 12,400 ± 60 by Beta Analytic Inc. and 12,275 ± 55 by Oxford University. Note that general mammalian primer pair that targets a small fragment of the 16S ribosomal RNA was negative for 1374-5/5D-31-2…”

 

Precluding positive DNA evidence for an herbivore (or any other mammal besides human) source for coprolite sample 1374-5/5D-31-2.

 

There are 26 coprolites from Paisley Caves identified as human, 7 are AMS dated older than Clovis (> 11,050 14C yr BP per Waters and Stafford 2007).

 

This statement in the paper sums up the authors' view concerning older than Clovis occupation of the Americas:

 

“…Although some claims have been made for pre-Clovis sites in North (e.g., Adovasio et al., 1999; Gilbert et al., 2008; Falk, 2004; Jenkins et al., 2012) and South America (Dillehay, 2000; Guidon, 1989; Waters and Stafford, 2007), the putative earliest sites that are typically mentioned are still controversial (Fiedel, 2000; Kelly, 2003). The first clear evidence of occupation appears much later and is related to the Clovis complex _11.000 to 10.800 14C yr BP (Waters and Stafford, 2007)…”

 

 

> BTW what are your thoughts on "Monte Verde" and

> the "Kelp Highway" frankly I see no impediments at

> all to either one.  Looking at Google earth and

> Monte Verde's present position I can see no major

> geographical barriers to entering the new world

> after all we're talking about bands of people that

> maybe numbered 25 to 50 people or so and there had

> to dozens to hundreds of different way to make it

> into the new world.   

 

The vast majority of archeologists agree with Goebel et al., 2008:

 

“…Perhaps the best candidate is the Monte Verde site (Chile), which

contains clear artifacts in a sealed context and is dated to 14.6 ka (58).

Despite criticism (59), its acceptance by most archaeologists means synchronous

and possibly earlier sites should exist in North America…”

 

mtDNA evidence is concordant with a Pacific coast migration (See Hg B ):

 

http://britam.org/MtDNAWorld.jpg

 

Note the lack of mtDNA B in northern North America and Siberia.

 

LGM sea levels:

 

http://emvc.geol.ucsb.edu/3_downloads/M2IceAge/aDeglacNoAmMovie/NoAmDeglaciation.mov

 

Distribution of stemmed projectiles in the Americas, during and before Clovis times, extends from Oregon to the tip of Chile, near the Straits of Magellan:

 

http://uonews.uoregon.edu/sites/uonews1.wc-sites.uoregon.edu/files/uploads/images/WS-bases-UOsite_0.jpg

 

http://upload.wikimedia.org/wikipedia/commons/b/b8/Puntas_cola_de_pescado_Cueva_Fell.jpg

 

In Siberia, the northern most dated stemmed points are found at The Ushki Site on the Kamchatka Peninsula:

 

http://csfa.tamu.edu/gallery/ushki-10.jpg

 

http://0.tqn.com/d/archaeology/1/0/_/J/1/potter1HR.jpg

 

“…Here we present four new radiocarbon dates (and two previously unpublished dates) on curated charcoal from hearth features excavated at Ushki-1 more than 20 years ago. They indicate that these hearths and associated dwelling features date to about 13,000 cal BP. We now know 15 radiocarbon dates on charcoal from a variety of features and profiles across Ushki-1 and Ushki-5 that indicate the age of layer 7 is about 13,000 cal BP…”

 

http://csfa.tamu.edu/cfsa-publications/Goebel-JAS2010-37-2640.pdf

 

13,000 cal BP falls within the Clovis timeframe, suggesting a possible technological backflow from North America to Siberia.

 

 


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